Keller PM, Davison AJ, Low RS, Bennett CD, Ellis RW (1986) Identification and structure of the gene encoding gpII, a major glycoprotein of varicella-zoster virus. Johnson DC, Frame MC, Ligas MW, Cross AM, Stow ND (1988) Herpes simplex virus immunoglobulin G Fc-receptor activity depends on a complex of two viral glycoproteins, gE and gI. Grose C, Litwin V (1988) Immunology of the varicella-zoster virus glycoproteins. Grose C, Edwards DP, Friedrichs WE, Weigle KA, McGuire WL (1984) Varicellazoster virus specific gp 140: a highly immunogenic and disulfide linked structural glycoprotein. Gershon JM, Palade GE (1983) Protein blotting: principles and applications. J Virol 56: 333–336Įing BR, Kühn JE, Braun RW (1989) Neutralizing activity of antibodies against the major herpes simplex virus type 1 glycoproteins. J Med Virol 16: 147–162Įdson CM, Hosler BA, Respess RA, Waters DJ, Thorley-Lawson DA (1985) Cross-reactivity between herpes simplex virus glycoprotein B and a 63,000-Dalton varicella-zoster virus envelope glycoprotein. J Gen Virol 65: 1839–1843Įberle R, Mou SW, Zaia JA (1985) The immune response to herpes simplex virus: comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes implex virus type 1 infections. J Infect Dis 157: 882–888Įberle R, Mou SW, Zaia JA (1984) Polypeptide specificity of the early antibody response following primary and recurrent genital herpes simplex virus type 2 infections. J Gen Virol 67: 1759–1816ĭubey L, Steinberg SP, LaRussa P, Oh P, Gershon AA (1988) Western blot analysis of antibody to varicella-zoster virus. Virol 133: 301–314ĭavison AJ, Scott JE (1986) The complete DNA sequence of varicella-zoster virus. J Virol 61: 1125–1135īzik DJ, Fox BA, DeLuca NA, Person S (1984) Nucleotide sequence specifying the glycoprotein gene gB of herpes simplex virus type 1. Analysis of sera from individuals with previous HSV and/or VZV infection showed the presence of antibodies crossreactive between HSV-1 gB and VZV gp-II in 3 out of 30 sera tested.īalachandran N, Oba DE, Hutt-Fletcher LM (1987) Antigenic cross-reactions among herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus. In contrast, no crossreactive antibodies were found in sera of HSV-seronegative patients with acute primary VZV infection (0/6) or in sera from individuals with acute recurrent HSV or VZV infection (0/12). The frequency of human IgG antibodies crossreactive between HSV-1 gB and VZV gp-II was highest in HSV-seropositive patients experiencing an acute primary VZV infection (4 out of 5 sera tested). Crossreaction of human IgG antibodies among other structural and nonstructural viral proteins, however, was not detected. Using antibody fractions purified on HSV- and VZV-coated affinity chromatography columns and by preadsorption of sera with HSV and/or VZV lysates a cross-reactivity between HSV-1 gB and VZV gp-II was demonstrated. Prospective evaluation of an algorithm incorporating HSV-1 serostatus found that 11 of 70 persons with indeterminate HSV-2 ELISAs were Western blot-positive.Ĭlinicians should consider selectively using a higher index value to define Focus ELISA HSV-2 positivity based on either HSV-1 serostatus or clinical circumstances.The specificity and prevalence of human IgG antibodies crossreactive between HSV-1 (ANG) and VZV (Ellen) was examined in immunoblots. Selectively raising the ELISA index value defining HSV-2 positivity from >1.1 to >or=3.0 either among HSV-1-positive men or among those without a history or clinical evidence of genital lesions increased the PPV to >or=93%. Western blot positivity was more common in men without herpes simplex virus type 1 (HSV-1) antibody than in those with HSV-1 antibody (93% vs 76%, P = 0.02) and in men with a history or clinical evidence of genital lesions (88% vs 80%, P = 0.30). Ninety-one (84%) of 108 HSV-2 ELISA-positive sera tested HSV-2 Western blot-positive. HSV-2 Western blots were then prospectively performed on sequential sera with indeterminate HSV-2 ELISAs. HSV-2 Western blots were performed on sera from male sexually transmitted disease clinic patients testing HSV-2 ELISA-positive and used to define a new class of indeterminate HSV-2 ELISA result. The objective of this study was to define the positive predictive value (PPV) of the Focus herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assay (ELISA) in a low HSV-2 prevalence population and to develop a new test interpretation algorithm.
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